Cell Based Assays
Invitrogen™ Dextran, Tetramethylrhodamine, 40,000 MW, Neutral
Rigorous methods for removing as much unconjugated dye as practical, followed by an assay of dextran conjugates by thin-layer chromatography to help ensure the absence of low molecular weight contaminants
Invitrogen™ JC-9 Dye (Mitochondrial Membrane Potential Probe)
Mitochondrial membrane potential probe
Invitrogen™ Dextran, Fluorescein and Tetramethylrhodamine, 70,000 MW, Anionic
Rigorous methods for removing as much unconjugated dye as practical, followed by an assay of dextran conjugates by thin-layer chromatography to help ensure the absence of low molecular weight contaminants
Invitrogen™ Dextran, Alexa Fluor™ 555; 10,000 MW, Anionic, Fixable
Rigorous methods for removing as much unconjugated dye as practical, followed by assay of dextran conjugates by thin-layer chromatography to help ensure absence of low molecular weight contaminants
Invitrogen™ Dextran, Tetramethylrhodamine, 2,000,000 MW, Lysine Fixable
Labeled dextrans are hydrophilic polysaccharides most commonly used in microscopy studies to monitor cell division, track the movement of live cells, and to report the hydrodynamic properties of the cytoplasmic matrix.
Invitrogen™ Dextran, Oregon Green™ 488; 10,000 MW, Anionic, Lysine Fixable
Rigorous methods for removing as much unconjugated dye as practical, followed by assay of dextran conjugates by thin-layer chromatography to help ensure absence of low molecular weight contaminants
Invitrogen™ eBioscience™ JC-1 Mitochondrial Membrane Potential Dye
Used to determine mitochondrial membrane potential in flow cytometry and fluorescent microscopy. Dye selectively enters mitochondria where it reversibly changes color as membrane potentials increase. Property due to the reversible formation of JC-1 aggregates upon membrane polarization.
Invitrogen™ eBioscience™ Fura-2 AM Dye
Imaging dye that binds to free Ca2+. Fura-2 is the preferred dye for ratiometric imaging microscopy with digital image analysis, especially when altering excitation wavelengths is more practical than detecting multiple emission wavelengths.
Invitrogen™ eBioscience™ Calcium Sensor Dye eFluor™ 514
Membrane-permeable dye used to monitor changes in intracellular free calcium concentrations in the cell. Once eFluor™ 514 Calcium Sensor Dye enters the cell, cellular esterases cleave the AM group yielding a membrane-impermeable dye fluorescing at ≈520nm.
Invitrogen™ CellVue Diluent C (For CellVue™ Cell Labeling Kits)
Required for the dilution of the dyes in the CellVue™ Cell Labeling Kits. Please refer to the specific labeling kit for instructions of use.
Invitrogen™ Histone H1 From Calf Thymus, Alexa Fluor™ 488 Conjugate
Labeled with the bright and photostable dye
Invitrogen™ BCECF, AM (2',7'-Bis-(2-Carboxyethyl)-5-(and-6)-Carboxyfluorescein, Acetoxymethyl Ester)
For measuring changes in the cytosolic pH of most cells
Invitrogen™ Lipopolysaccharides From Escherichia coli Serotype 055:B5, Alexa Fluor™ 568 Conjugate
Bright, orange-red fluorescent conjugate can be used to follow lipopolysaccharide binding, transport and cell-internalization processes
Invitrogen™ LIVE/DEAD™ Fixable Scarlet (723) Viability Kit, for 633 nm excitation
The LIVE/DEAD Fixable viability kits allow for clear determination of live and dead cells, enabling more precise analysis with both conventional and spectral flow cytometry.